Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability

In the present study, three experiments were designed to identify most appropriate technique for freezing rabbit semen. Experiment 1 aimed determine optimal levels of dimethyl sulfoxide (DMSO) contents in medium and their effects on individual bucks. Semen ejaculates each buck (n=15 bucks) mixed split into portions extension with a containing varying concentrations DMSO (0.75, 1.0, 1.4 M). Diluted semen samples packaged 0.25 mL straws suspended above liquid nitrogen (LN) 10 min, then dipped LN. A few days after freezing, post-thaw evaluation was assessed, according results, six bucks an extender 0.75 M used 2 3. experiment 2, pooled from 6 divided two packaging straw sizes (0.25 0.50 mL). Each size five groups at different heights LN (2, 4, 6, 8, cm) minutes before being preserved 3, four dilution bovine serum albumin (BSA; 0, 2.5, 5.0, 7.5 mg/mL). 4 cm freezing. Pre-freezing post-thawing, evaluated quality. Results showed that had higher significant values sperm motility, longevity, acrosome integrity plasma membrane permeability. Bucks’ individuality integrity. interaction recorded between bucks’ longevity. package no parameters. frozen significantly better BSA 5 mg/mL improved recovery rates seem be more adequate cryopreservation. The level differs bucks, as longevity is affected. enhanced obtained will need further investigation confirmed field.